INTRODUCTION
Star Staining fluorescent-labeled products represent a new generation of tools developed with ACROBiosystems’ “Star Standard” quality site-specific labeling technology. These innovative products are specifically designed to excel as a Star Tool For Car detection, particularly for monitoring CAR-T cells in clinical trials.
Fluorescent-labeled proteins are indispensable in the realm of CAR expression detection within both research and clinical settings, especially when employing flow cytometry. These proteins, pre-conjugated with vibrant fluorescent dyes, simplify CAR expression detection through a streamlined one-step staining process that minimizes background noise. However, the creation of high-caliber fluorescent-labeled proteins is technically demanding, resulting in limited commercial availability.
The conjugation technique employed is a critical factor determining the quality of fluorescent-labeled proteins. Traditional chemical labeling, the most common method, offers simplicity in covalently attaching fluorescent dyes to proteins in a non-specific manner. However, this randomness can lead to product heterogeneity and potentially compromise protein bioactivity by inadvertently blocking active sites. In contrast, the advanced site-specific labeling technology ensures fluorescent dyes are attached at precise locations on the protein, away from active sites. This targeted approach guarantees product uniformity and preserves protein function, making these “Star Staining” products a superior star tool for car development, offering enhanced specificity and sensitivity.
PRODUCT FEATURE
Traditional Chemical Labeling
Illustration of traditional chemical labeling, showing random dye attachment potentially disrupting protein structure and function.
Traditional chemical labeling methods suffer from several drawbacks that impact the quality and reliability of fluorescent-labeled proteins:
- Non-selective random labeling: Dyes attach randomly, altering the protein’s natural structure.
- Active site blockage: Labeling may occur at or near active sites, reducing bioactivity.
- Aggregation risk: Changes in protein hydrophilicity can lead to aggregation.
- Batch inconsistency: High variability between different production batches.
New-generation Site-specific Labeling
Diagram illustrating site-specific labeling, highlighting precise dye attachment away from critical functional regions of the protein.
ACROBiosystems’ new-generation site-specific labeling technology overcomes the limitations of traditional methods, providing significant advantages:
- Specific tag labeling: Dyes are attached to a specific tag, distant from protein active sites.
- Natural conformation maintenance: Preserves the protein’s natural structure and post-translational modifications.
- Efficient bio-orthogonal technology: Ensures clean and efficient labeling.
- High batch consistency and uniformity: Minimizes batch-to-batch variations, ensuring reproducible results. This consistency makes Star Staining products a dependable star tool for car research and clinical applications.
STAR STAINING FEATURES
ACROBiosystems is dedicated to developing gold-standard detection reagents to advance CAR-T cell therapy. Years of dedicated research and technical expertise have culminated in the establishment of the “Star Staining” site-specific labeling platform. This platform underpins a range of exclusive fluorescent-labeled proteins characterized by “Star Standard” quality. “Star Staining” products are powerful tools for detecting and monitoring CAR-T cells in clinical trials, truly a star tool for car T-cell therapy monitoring.
Key features of Star Staining products, establishing them as a star tool for car analysis:
- Natural Bioactivity Preservation: Utilizes new-generation site-specific labeling to maintain the protein’s inherent biological activity, crucial for accurate CAR detection.
- High Specificity and Sensitivity: Rigorously verified by flow cytometry, ensuring precise and reliable CAR-T cell detection with minimal false positives.
- No Non-specific Binding to Non-transduced PBMCs: Minimizes background noise by eliminating non-specific binding, leading to cleaner and more accurate data in CAR-T cell assays.
- High Batch-to-Batch Consistency and Uniformity: Guarantees reproducible results across different lots, essential for reliable and consistent performance in long-term studies and clinical applications. This consistency makes Star Staining a dependable star tool for car research.
HOT PRODUCTS
Star Staining products are available with a variety of fluorescent labels to suit diverse experimental needs:
- FITC (Fluorescein isothiocyanate)
- PE (Phycoerythrin)
- APC (Allophycocyanin)
- Alexa Fluor 647
- Alexa Fluor 555
- Alexa Fluor 488
| Molecule | Cat. No. | Product Description | Preorder/Order |
|---|---|---|---|
(Note: Product list table from original article is incomplete and would require product-specific details to be fully populated. In a real scenario, this table would be filled with actual product names, catalog numbers, descriptions, and order links.)
DATA DISPLAY
High Purity
Star Staining FITC-labeled Human BCMA demonstrates exceptional purity, exceeding 90%.
SDS-PAGE analysis of FITC-Labeled Human BCMA, His Tag (Cat. No. BCA-HF2H3) under reducing conditions (R). Coomassie Blue staining reveals high purity, greater than 90%.
SEC-MALS analysis confirming the high purity (>90%) and molecular weight (24-34 kDa) of FITC-Labeled Human BCMA, His Tag (Cat. No. BCA-HF2H3).
High Bioactivity
Star Staining products exhibit superior binding activity compared to competitors. This high bioactivity confirms Star Staining as a potent star tool for car target engagement studies.
FACS analysis comparing the binding activity of FITC-Labeled Human BCMA and CD19 proteins from ACROBiosystems and competitor vendors. ACRO’s Star Staining FITC-Labeled Human BCMA (Cat. No. BCA-HF2H3) and CD19 (Cat. No. CD9-HF2H3) demonstrate significantly higher binding activity.
Activity comparison of Alexa Fluor 647-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HA2H5) against a competitor product, showing superior performance.
Activity comparison of Alexa Fluor 488-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HA2H9) against a competitor product, demonstrating enhanced activity.
Star Staining PE-CD19 or APC-CD19 proteins provide high-sensitivity detection of CD19 CAR expression using flow cytometry, further solidifying their position as a star tool for car T-cell analysis.
FACS analysis of Anti-CD19 CAR-293 cells stained with PE-Labeled Human CD19 (20-291) Protein (Cat. No. CD9-HP2H5), His Tag star staining, and a negative control. PE signal indicates binding activity.
FACS analysis of Anti-CD19 CAR-293 cells stained with APC-Labeled Human CD19 (20-291) Protein (Cat. No. CD9-HA2H9), His Tag star staining, and a negative control. APC signal demonstrates binding activity.
No Non-specific Binding to Non-transduced PBMCs
Star Staining products minimize background noise, showing no non-specific binding to non-transduced PBMCs and exhibiting higher specific binding than competitors. This feature is crucial for accurate and reliable CAR-T cell detection, making them a premier star tool for car T-cell research.
FACS analysis comparing non-specific binding to non-transduced PBMCs between Acro’s PE-Labeled Human BCMA Protein and a competitor product. 5e5 non-transduced PBMCs were stained with PE-Labeled Human BCMA Protein and anti-CD3 antibody. FITC signal assessed CD3+ T cell expression, and PE signal evaluated non-specific binding.
Comparison of non-specific binding to non-transduced PBMCs between Acro’s FITC-Labeled Human BCMA Protein and a competitor. 5e5 non-transduced PBMCs were stained with FITC-Labeled Human BCMA Protein and anti-CD3 antibody. PE signal measured CD3+ T cell expression, and FITC signal assessed non-specific binding.
Maintain Natural Bioactivity
Star Staining technology ensures high binding capacity is maintained even after conjugation, as confirmed by both FACS and SPR analysis. This preservation of bioactivity is a key attribute of Star Staining as a star tool for car target analysis.
FACS analysis evaluating the binding activity of Human BCMA before and after FITC labeling. Results show comparable binding activity between FITC-Labeled BCMA (Cat. No. BCA-HF2H3) and unconjugated Human BCMA.
SPR analysis (Biacore T200) comparing the binding affinity of Human BCMA before and after FITC labeling. Results demonstrate similar affinity between FITC-Labeled (Cat. No. BCA-HF2H3) and unconjugated Human BCMA, His Tag.
High Batch-to-Batch Consistency
FACS verification confirms high batch-to-batch consistency in the binding activity of different lots of Star Staining FITC/Alexa Fluor-555/PE-labeled Human BCMA protein, assuring researchers of reliable and reproducible results when using Star Staining as their star tool for car research.
Flow cytometry analysis assessing the binding activity of different lots of FITC-Labeled Human BCMA (Cat. No. BCA-HF2H3) , AF555-Labeled Human BCMA (Cat. No. BCA-HA2H6), and PE-Labeled Human BCMA (Cat. No. BCA-HP2H7) against anti-BCMA CAR-293 cells. Results demonstrate excellent batch-to-batch consistency.
High Stability
Star Staining products maintain their performance even under stress conditions. Stability testing confirms that PE-Labeled Human BCMA and FITC-Labeled Human BCMA remain stable at 25℃ for 48 hours and through freeze-thaw cycles, highlighting their robustness as a dependable star tool for car research.
Stability assessment of PE-Labeled Human BCMA (Cat. No. BCA-HP2H7) and FITC-Labeled Human BCMA (Cat. No. BCA-HF2H3) after 48 hours at 25℃ and freeze-thaw cycles, showing no performance degradation.
More CAR-T Related Products
Explore our comprehensive range of products supporting CAR-T cell therapy research and development:
Learn More about CAR-T TargetsLearn More about Fluorescent-labeled ProteinsLearn More about Anti-FMC63 scFv antibodiesLearn More about CD19 Proteins
REFERENCES
[1] Abbasov ME, Kavanagh ME, Ichu TA, et al. A proteome-wide atlas of lysine-reactive chemistry [published online ahead of print, 2021 Sep 9]. Nat Chem. 2021;10.1038/s41557-021-00765-4.
[2] Xu L, Kuan SL, Weil T. Contemporary Approaches for Site-Selective Dual Functionalization of Proteins. Angew Chem Int Ed Engl. 2021;60(25):13757-13777.
[3] Devabhaktuni A, Lin S, Zhang L, et al. TagGraph reveals vast protein modification landscapes from large tandem mass spectrometry datasets. Nat Biotechnol. 2019;37(4):469-479.
